Ultrastructural changes in oocytes during folliculogenesis in domestic mammals

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Ultrastructural changes in oocytes during folliculogenesis in domestic mammals

The ultrastructural analysis of oocytes and ovarian follicles has been used to evaluate the effects of assisted reproductive techniques, such as cryopreservation or in vitro oocyte maturation. It also benefits the understanding of such complex mechanisms that occur during folliculogenesis. From the beginning of primordial follicles growth until oocyte maturation in preovulatory follicles oocyte...

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Cumulus cell expansion and ultrastructural changes in in vitro matured bovine oocytes under heat stress

Cumulus-oocyte complexes (COCs) from cows were matured under normal (38.5°C) and elevated temperatures (41°C) simulating heat stress and their maturation was assessed based on measurement of cumulus expansion in both groups. There was a significant reduction (P

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Ultrastructural Changes of Sheep Oocytes Follow Vitrification by Different Methods and In Vitro Maturation

Purpose: Our aim was determination of the sheep oocytes ultrastructural changes follow vitrification and in vitro maturation. Materials and Methods: Good quality isolated cumulus-oocyte complexes (COCs) were randomly divided into non-vitrified control, conventional straw, cryotop and solid surface vitrification groups. In the conventional and cryotop methods the vitrified COCs were plunged dire...

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DNA methylation reprogramming occurs during mammalian gametogenesis and embryogenesis. Sex-specific DNA methylation patterns at specific CpG islands controlling imprinted genes are acquired during this window of development. Characterization of the DNA methylation dynamics of imprinted genes acquired by oocytes during folliculogenesis is essential for understanding the physiological and genetic...

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ژورنال

عنوان ژورنال: Journal of Ovarian Research

سال: 2014

ISSN: 1757-2215

DOI: 10.1186/s13048-014-0102-6